Does Biostimulation Accelerate Algae Growth?

Algae and bacteria are the fastest growing organisms on our planet. A single algae cell can produce over one million daughter cells in one day. Is this fast enough? All algae producers are limited by sustainable biomass accumulation. Growth rates impact both capital and operational costs. A 20% increase in growth rate could give some growers a 30% increase in revenue.

John Ericsson, CEO, AlgaStar Inc. presented at the 2013 Algae Biomass Summit in Orlando a session titled: Increasing Algal Biomass Production Using Electromagnetic Bio-Stimulation. Ericsson reported that their research team used biological simulation to achieve a 300% increase in algae growth rate over normal conditions. Research conducted at AlgaStar and recently initiated at Los Alamos National Laboratory has begun to map the conditions under which biostimulation enhances growth rate and metabolism for several biological cultures with microwave energy.

Biological stimulation research began in the 1920’s, re-emerged in the 1950’s with the space race and continues today. Music and wine grapes provide an interesting parallel track. If music makes better wine, might sound, electronic or microwaves similarly stimulate algae?

Music and Wine Grapes

Wine grape growers in Italy, South Africa, France, Washington and California play classical music to the vineyards to enhance growth rates and sugar production. The music equipment company Bose has sponsored music and wine research by providing their excellent speakers for placement in wine vineyards. The effect of sound on plants depends on frequency, intensity and exposure. In 2001, Chinese researchers found that low-frequency sound does not change cell structure but does activate enzymes, increases cell-membrane fluidity and promotes DNA replication and cell cycling.

Wine grape farmer Carlo Cignozzi equates music to fertilizer for grapes in his Al Paradiso di Frassina vineyard in Tuscany. He favors classical music such as Mozart and Tchaikovsky. He says music scares off animals that feed on young grapevines, as well as parasites, molds and bacteria. He calls music fantastic because bunches closest to the music grow grow faster, mature 10 days earlier and have higher the alcohol content. The results on his vineyard have been so impressive that Florence University has launched a research to scientifically validate them. Researchers will examine music versus silence and the response of the vines to different sound frequencies.

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The DeMorgenzon web site proudly announces: “We pipe Baroque music through our vineyards 24×7 and believe that the power of music positively influences the ripening process.” The owner, Hylton Appelbaum in South Africa notes that his vines respond to a very particular style of classical music. Rock, pop, rap, techno and jazz do not have the same effect. His vines enjoy harmonious and melodious classical or wordless baroque music. He has observed that music stimulates the vines to grow more vigorously and to have more vitality. His vines exposed to music have a bigger surface area of leaves, which increases photosynthesis. Enhanced photosynthesis results in faster growth with higher sugar and flavor accumulation in the grapes.

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A French physicist and musician, Joel Sternheimer filed a patent application in 1994 covering his assertion that playing the appropriate tune stimulates protein synthesis. His model includes melodies for cytochrome oxidase and cytochrome C proteins involved in respiration.

Researchers at South Korea’s National Institute of Agricultural Biotechnology found classical music triggered a response in two specific genes (rbcS and Ald) in rice plants. Scientists at Trakya University in Turkey found relaxing, calming and mentally invigorating music had a positive effect on root growth in onions during germination. Another study in Chemosphere examined music modeling and found calm music improved boron uptake in plants.

Algae Biostimulation

Biological stimulation from electromagnetic fields and/or microwaves offers a novel technology that can accelerate algae growth substantially compared with natural sunlight. Laboratory tests at AlgaStar, Inc. and research collaborators at the University of Western Ontario, (UWO) have proven the biostimulation concept but considerably more research is needed. Additional research efforts are now funded for AlgaStar with Los Alamos National Laboratory. Additional grant applications and research sponsor funding will include Dr. Bruce Rittmann’s lab in the Biodesign Institute at ASU, the world class AzCATI Test Bed at ASU, NanoVoltaics, UWO and others.

The AlgaStar algae production and biostimulation system integrates two types of electromagnetic energy. The first is a millitesla generator and the second a millimeter microwave generator that radiates spontaneous growth energy into large volumes of algae biomass. The research teams have demonstrated that electromagnetic energy waves can provide an increase in algae biomass and its corresponding lipid oil production by up to 300%.

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AlgaStar’s patented 4500 gallon SolarMagnatronTM, biomass production system uses an automated biosystem controller, (ABC) which optimizes biomass production. The ABC can control the biostimulation EMF frequency and amplitude to optimize the algae growth cycle and substantially reduces the risk of culture crash. The ABC controls light, temperature, pH, nutrients and delivery of carbon dioxide to achieve optimal productivity. The ABC will allow the grower to harvest on precisely the schedule that maximizes growth such as 1% of the biomass every 15 minutes or 50% once a day. The ABC transforms the SolarMagnatronTM system into an adaptable microcrop platform that can mimic ideal growing conditions for many algae species as well as other microorganisms.

solarmagnatron

The SolarMagnatronTM uses light very efficiently. The design allows continuous growth during daylight hours by maximizing the use of natural sunlight. The system is constructed primarily fiberglass, acrylic and flat glass. During the non-daylight hours, special domed acrylic lenses and flat-panel glass reactors containing high-efficiency florescent and LED lights produce artificial sunlight at specific wavelengths and power levels that optimize algae photosynthesis.

The gravity flow and air lift circulation systems uses very little energy. AlgaStar is working on a PV DC power system that would enable siting off-grid.

AlgaStar’s SolarMagnatron ™ production platform is economically built, ecologically sensitive and sustainable as it minimizes water consumption and evaporation. Production of oils, protein and carbohydrates per kilogram require less than 10% of the water consumed by field crops. Production of specialty products such as Omega 3 fatty acids require less than 1% of the water consumed by field crops. The system reduces costs further by allowing growers to use non-potable water such as brine, brackish, waste or ocean. An integrated UV and Ozone unit sanitizes incoming water as well as recycled water after biomass harvest. As an enclosed system, genetically modified organisms can be grown with minimal risk to the natural environment.

Path Forward

AlgaStar’s SolarMagnatron™ with EMF biostimulation methods offers substantial value to the algae and other microcrop industries for increasing biomass production The future research extension and validation proposed by AlgaStar with Los Alamos National Laboratory, the Biodesign Institute at ASU, the AzCATI Test Bed at ASU, NanoVoltaics and UWO will provide a fascinating examination and analysis of EMF biostimulation with algae and other high value biochemical and energy producing microorganisms.

Among the questions remaining for biostimulation, top priority seem to include:

1. How, why and where does EMF biostimulation work?
2. What is the pathway that enables faster mitosis (cell division)?
3. Are there any positive vs negative impacts on the organism from EMF biostimulation?
4. What algae species and other microorganisms are most receptive to biostimulation for increasing biomass for food, feed, biofuel, fertilizers, pharmaceuticals, nutraceuticals and medicines?
5. How does EMF power levels and frequency matter in the EMF biostimulation process?
6. What types of EMF are best suited for biostimulation?
7. How does the energy wave source matter – either electromagnetic, sound or microwaves?
8. Can laboratory conditions for biostimulation be replicated in full-scale production settings, including open–pond, hybrid and closed systems?
9. Can biostimulation create substantially higher production target compounds as well as total biomass?
10. How can biostimulation be matched to optimize various growth stages in many organisms, which could be used to increase production of drugs, in the event of a pandemic flue event or in many other applications to support the survival of mankind?

EMF biostimulation offers the promise of higher productivity for algae and other valuable microorganisms. The current research path will offer fascinating opportunities for enhancing commercial production of algae growth and with many other valuable biochemicals.

Original blog posted by Mark Edwards, Ph.D.

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